Survey of Ophthalmology
Volume 45 , Pages S268-S272 , May 2001

In Situ Detection of Apoptosis in Normal Pressure Glaucoma: A Preliminary Examination

  • Nadine A Tatton, PhD

      Affiliations

    • Department of Neurology, Mt. Sinai School of Medicine/New York University, New York, NY USA
    • Corresponding Author InformationReprint address: Nadine A. Tatton, PhD, Department of Neurology, Box 1137, Annenberg Bldg. Rm 20-02, Mt. Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029.
    • ,
  • Gulgun Tezel, MD

      Affiliations

    • Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, MO USA
    • ,
  • Stephanie A Insolia, MS

      Affiliations

    • Department of Neurology, Mt. Sinai School of Medicine/New York University, New York, NY USA
    • ,
  • Sarah A Nandor, BS

      Affiliations

    • Department of Neurology, Mt. Sinai School of Medicine/New York University, New York, NY USA
    • ,
  • P.Deepak Edward, MD

      Affiliations

    • Department of Ophthalmology and Visual Sciences, University of Illinois Eye Center, Chicago, IL, USA
    • ,
  • Martin B Wax, MD

      Affiliations

    • Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, MO USA

References 

  1. Baumgartner-Parzer SM, Wagner L, Pettermann M, et al.  High-glucose–triggered apoptosis in cultured endothelial cells. Diabetes. 1995;44:1323–1327
  2. Berkelaar M, Clarke DB, Wang YC, et al.  Axotomy results in delayed death and apoptosis of retinal ganglion cells in adult rats. J Neurosci. 1994;14:4368–4374
  3. Carlile GW, Chalmers-Redman RM, Tatton NA, et al.  Reduced apoptosis after nerve growth factor and serum withdrawal (conversion of tetrameric glyceraldehyde-3-phosphate dehydrogenase to a dimer). Mol Pharmacol. 2000;57:2–12
  4. Darzynkiewicz Z, Traganos F. Measurement of apoptosis. Adv Biochem Eng Biotechnol. 1998;62:34–73
  5. Dypbukt JM, Ankarcrona M, Burkitt M, et al.  Different prooxidant levels stimulate growth, trigger apoptosis, or produce necrosis of insulin-secreting RINm5F cells. The role of intracellular polyamines. J Biol Chem. 1994;269:30553–30560
  6. Gavrieli Y, Sherman Y, Ben-Sasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol. 1992;119:493–501
  7. Gupta N, Weinreb RN. New definitions of glaucoma. Curr Opin Ophthalmol. 1997;8:38–41
  8. Ishitani R, Tanaka M, Sunaga K, et al.  Nuclear localization of overexpressed glyceraldehyde-3-phosphate dehydrogenase in cultured cerebellar neurons undergoing apoptosis. Mol Pharmacol. 1998;53:701–707
  9. Kass GE, Eriksson JE, Weis M, et al.  Chromatin condensation during apoptosis requires ATP. Biochem J. 1996;318:749–752
  10. Kerr JF, Wyllie AH, Currie AR. Apoptosis (a basic biological phenomenon with wide-ranging implications in tissue kinetics). Br J Cancer. 1972;26:239–257
  11. Kerrigan LA, Zack DJ, Quigley HA, et al.  TUNEL-positive ganglion cells in human primary open-angle glaucoma. Arch Ophthalmol. 1997;115:1031–1035
  12. Li Y, Schlamp CL, Nickells RW. Experimental induction of retinal ganglion cell death in adult mice. Invest Ophthalmol Vis Sci. 1999;40:1004–1008
  13. Oberhammer F, Fritsch G, Schmied M, et al.  Condensation of the chromatin at the membrane of an apoptotic nucleus is not associated with activation of an endonuclease. J Cell Sci. 1993;104:317–326
  14. Oberhammer F, Wilson JW, Dive C, et al.  Apoptotic death in epithelial cells (cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation). EMBO J. 1993;12:3679–3684
  15. Okisaka S, Murakami A, Mizukawa A, Ito J. Apoptosis in retinal ganglion cell decrease in human glaucomatous eyes. Jpn J Ophthalmol. 1997;41:84–88
  16. Rossiter JP, Riopelle RJ, Bisby MA. Axotomy-induced apoptotic cell death of neonatal rat facial motoneurons (time course analysis and relation to NADPH-diaphorase activity). Exp Neurol. 1996;138:33–44
  17. Sahara S, Aoto M, Eguchi Y, et al.  Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature. 1999;401:168–173[see comments]
  18. Sane AT, Bertrand R. Distinct steps in DNA fragmentation pathway during camptothecin-induced apoptosis involved caspase-, benzyloxycarbonyl- and N-tosyl-L- phenylalanylchloromethyl ketone-sensitive activities. Cancer Res. 1998;58:3066–3072
  19. Sun DY, Jiang S, Zheng LM, et al.  Separate metabolic pathways leading to DNA fragmentation and apoptotic chromatin condensation. J Exp Med. 1994;179:559–568
  20. Tabor S, Struhl K, Scharf SJ, Gelfand DH: Enzymatic manipulation of DNA and RNA, in Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Strukl K (eds.) Current Protocols in Molecular Biology. J Wiley & Sons Inc 1997
  21. Tatton NA. Increased caspase 3 and bax immunoreactivity accompany nuclear GAPDH translocation and neuronal apoptosis in Parkinsons disease. Exp Neurol. 2000;166:29–43[In Process Citation]
  22. Tatton NA, Kish SJ. In situ detection of apoptotic nuclei in the substantia nigra compacta of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated mice using terminal deoxynucleotidyl transferase labelling and acridine orange staining. Neuroscience. 1997;77:1037–1048
  23. Tatton NA, Maclean-Fraser A, Tatton WG, et al.  A fluorescent double-labeling method to detect and confirm apoptotic nuclei in Parkinsons disease. Ann Neurol. 1998;44:S142–S148
  24. Walker PR, Leblanc J, Carson C, et al.  Neither caspase-3 nor DNA fragmentation factor is required for high molecular weight DNA degradation in apoptosis. Ann NY Acad Sci. 1999;887:48–59
  25. Wyllie AH, Kerr JF, Currie AR. Cell death (the significance of apoptosis). Int Rev Cytol. 1980;68:251–306
  26. Zhizhina GP, Korobko VG, Alesenko AV: [Correlation of DNA degradation, induced by tumor necrosis factor- alpha, with accumulation of sphingosine in the nucleus and peroxides in DNA]. Biokhimiia 59:1756-65, 1994

PII: S0039-6257(01)00204-1

Survey of Ophthalmology
Volume 45 , Pages S268-S272 , May 2001

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